Skip to main content

Services description

The Facility makes every effort to ensure:  

  • a high  level of equipment performance through maintenance schedule and regular monitoring,
  • accompanying users (feasibility study, training for hands-on use, interaction during the service provision, expertise),
  • raw data backup during the provision of service.

The Facility has an obligation of means and not an obligation of results. Therefore the Facility should not be held responsible for any mistake done by users or unexpected equipment failure.

Confidentiality: The facility undertakes not to disclose any information concerning the project except the user’s name, its unit of origin and the project title. Upon request the project title can be kept confidential.

 

Analyses

The samples are sent to the Facility under the form specified in the Detailed Analysis List or in the Service Form. (Detailed Analysis List)

lcms  webfig

Biochemical phenotyping of plant samples

  • Automated analyses on microplates: total proteins, starch, sucrose, glucose, fructose, malate, fumarate (under proviso), citrate, total free amino acids, glutamate, proline, chlorophylls a and b (from one extraction)
  • Quantification of amino acids by UHPLC-fluorimetry
  • Analysis of phenolics (HPLC-UV/Visible, LC-MS ion trap, LC-NMR)

Metabolomic profiling-Metabolome of plant samples 

  • Quantification of major polar metabolites by 1H-NMR of polar extracts
  • Acquisition and analysis of metabolomic fingerprints by 1H-NMR of polar extracts
  • Acquisition and analysis of metabolomic fingerprints by LC-QqTOF-MS or LC-Orbitrap-MS of semi-polar extracts
  • Lipid profiling-Lipidome of plant samples 

Lipid analysis (phospholipids, neutral lipids, sphingolipids…)

  • Analysis and quantification of fatty acids by GC-FID or GC-MS
  • Analysis and quantification of lipids by GC-FID, GC-MS or TLC
  • Analysis of waxes and lipid polymers by GC-MS and GC-FID
  • Acquisition and analysis of lipidomic profiles by MS/MS and LC-Qtrap-MS

Compound identification 

  • Structural analysis of polyphenols (using MS and NMR)

Metabolism 

  • Automated measurement of central-metabolism enzymes (glycolysis, TCA cycle, Calvin-pentose phosphates, sucrose metabolism, nitrogen assimilation)
  • Acquisition of spectra for the measurement of 13C isotopic enrichments on fractions, for metabolic flux measurement

Result reporting

The results are communicated to the user in printed or electronic format. Without any user’s answer within ten days from receipt, the results are considered as accepted and the service as closed. However, the Facility members remain at the disposal of users for any question and help in result valorization.


 

Open use of analytical equipment 

  • Training and technical support
  • Use of :
    •   robots and microplate readers (340 nm, 405 nm, 520 nm, 570 nm and 600 nm)
    •   several equipments for analyses of metabolites or lipids
    •   NMR spectrometers

 Approach

 Equipment designation

 High-density Metabolomics-Metabolic profiling
  • LCMS MicrOTOf-Q (Bruker)
  • UHPLC-Fluo-DAD (Dionex)
  • 500 MHz NMR spectrometer AVANCE III
  • pH meter for NMR metabolomic/metabolic profiling extracts (Mettler Toledo)
  • Robot Titration BTpH
  • UHPLC-LTQ/Orbitrap
 High-throughput metabolic phenotyping
  • Robots de pipetage Starlet (Hamilton)
  • Microplate readers (Safas)
  • Microplate reader Fluo Xenius (Safas)
  • Tissue Lyser II grinding system (Retsch)
  • Capper-decapper for screw cap tubes (Hamilton)
  • Broyeur Geno-grinder (2 equipments)
 Lipidomics
  • ADC2 (Camag)
  • UV lamp for visualization of TLC spots (Camag)
  • GC-FID system (Agilent) (2 equipments)
  • GC-MS system (Agilent)
  • UHPLC-LTQ/Orbitrap
 Polyphenols- Structural identification
  • 600 MHz NMR spectrometer (Avance III, Bruker) equipped with an LC and SPE interface
  • LC-MSn ion-trap system (Bruker)
  • UV-Vis Spectrometer CARY 300 (Varian)

 

Related services 

Expertise and analysis of results

 

Other services on request

Technological developments may be done on request
Contact us