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Plateforme Métabolome Bordeaux

Scientific Leader MOING Annick
Technical Leader DEBORDE Catherine
Platform address Plateforme Métabolome de Bordeaux - MetaboHUB
Centre INRA de Bordeaux,
Site de Villenave d'Ornon, IBVM CS20032
33140 Villenave d'Ornon
Contact : pmfb@bordeaux.inra.fr
Platform web site METABOLOME

Bordeaux Metabolome Facility provides equipments and expertise for the study of metabolome and metabolic fluxes. The facility is mostly dedicated to the study of plant or plant-derived products.

Equipment 

  • A 500 MHz NMR spectrometer (2001, 2011) and its environment 
  • A 600 MHz NMR spectrometer (2008) and its environment including a LC-NMR probe (2008)
  • An  LC-QqTOF-MS system (2007)
  • An ion-trap LC-MSn system (2007-2014)
  • A GC-MS system (2006)
  • LC-Qtrap-MS systems (2017)
  • An UHPLC-DAD system (2010)
  • An UHPLC-LTQ-Orbitrap-MS system (2014)
  • Robotized systems for high-throughput phenotyping with microplates (2009, 2014) 

Services

Metabolome, lipidome

  • Fingerprints or metabolic profiles with proton NMR or LC-MS,
  • Quantitative metabolomics : metabolic profiles with quantitative proton NMR or UHPLC-DAD,
  • Lipidomics: identification and quantification by HPTLC, LC-MS/MS, GC-FID or GC-MS of lipids or fatty acids,
  • Structural analysis of compounds using multidimensional NMR.

Metabolism & fluxome

  • High-throughput robotized measurement of metabolites and enzyme activities
  • Analysis of lipid metabolism pathways using labeling and HPTLC,
  • Measurement of isotopic enrichments after labeling with stable isotopes (1H and 13C-NMR) for the quantification of metabolic fluxes.

Related services

  • Extractions adapted to the chosen biochemical analysis, tissue or matrix,
  • Statistical analyses of metabolome data,
  • Handling and storage of the produced data and metadata (databases including MeRy-B, http://bit.ly/meryb ),
  • Personalized training of students and scientists (analytical and statistical),
  • Organization of workshops and scientific meetings.

Application domains

The Metabolome Facility supports studies of plant functional genomics,  genetics, pathology, ecophysiology and systems biology for regional, national and international research programs. It is also used for the study of plant-derived products. Most studied topics include:

  • acquisition and interpretation of metabolomic profiles,
  • biochemical phenotyping,
  • studies of the dynamics of metabolism,
  • functional analysis of lipid metabolism.

Technological developments 

  • Robotisation of sample grinding and weighing,
  • Obtention of rapid metabolic signatures by high-throughput 1H-NMR,
  • Ultra-fast 2D NMR and 13C NMR developments, in collaboration,
  • Quantitative MS-based analysis of intermediary compounds of central metabolism, 
  • Lipidomics of plant samples,
  • Transfer of high-throughput metabolic phenotyping to diverse models,
  • Identification of compounds using LC-SPE-NMR and multidimensional NMR,
  • Constitution of a library/database of reference compounds and corresponding spectra,
  • Bioinformatic tools for NMR spectra processing, handling and sharing,
  • Tools for data handling, sharing and mining.

Technological developments at Metabolome

Highlights 

  • Participation in several European projects each year.
  • Participation in several ANR projects each year, including "Biotechnology and Bioresource" projects.
  • Implication in the actions of the “Francophone Metabolomics and Fluxomics Network”, and “GERLI”.
  • Coordination of MetaboHUB INBS and participation in PHENOME INBS.
metabohub

Aims

  • Bordeaux Metabolome Facility, supported by three Institutes (INRA, CNRS and Bordeaux University), gathers equipments and expertise for the study of metabolism and make them available to the scientific community.
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  • The Facility performs adaptations and technological developments and designs and implements analytical strategies and bioinformatics tools for metabolomics, metabolic phenotyping, lipidomics and fluxomics.

  

  • The Facility supports studies of plant functional genomics, genetics, pathology, ecophysiology and systems biology for regional, national and international research programs. It is also used for the study of plant-derived products and for pharmacological approaches.
     
missions

 

  • The Facility welcomes and trains students and scientific visitors on the metabolomics, lipidomics and metabolic phenotyping technologies and tools.
     

training